Gastrointestinal infections due to consumption of contaminated seafood/shellfish or water are most commonly associated with, V.parahaemolyticus, V.vulnificus and , V.alginolyticus. Travel associated infection with V.cholerae can lead to severe illness (cholera) which may quickly become fatal if untreated.
Vibrio Species DNA
Vibrio Species DNA
Vibrio bacteria are pathogens which are found in water. There are a number of species which cause infection although cases are rare in Ireland.
Non-cholera bacteria such as V.parahaemolyticus, V.vulnificus, V.fluvialis can lead to several clinical manifestations which are generally mild and self-limiting. Symptoms include diarrhoea, abdominal pain and fever.
Infection due to V.cholera is characterised by profuse watery diarrhoea resulting in dehydration with fluid and electrolyte loss and is associated with a poor outcome.
The incubation period for Vibrio-associated diseases ranges from less 1 day to several days. An individual may be infectious for up to 14 days after contracting a Vibrio infection.
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Loose/liquid human faecal sample.
Enfer Medical
Tests/Assays
The test for Vibrio offered by Enfer Medical is carried out by real-time multiplexed PCR, as part of an extended gastrointestinal panel. The test is CE-IVD certified.
Bacteria | Viruses | Parasites |
Campylobacter | Adenovirus F40/41 | Cryptosporidium |
Clostridium difficile toxin A/B | Astrovirus | Cyclospora cayetanensis |
Plesiomonas shigelloides | Rotavirus A | Entamoeba histolytica |
Salmonella | Novovirus GI/GII | Giardia lamblia |
Vibrio | Sapovirus (Genogroups I, II, IV and V) | |
Yersinia enterocolitica | ||
Escherichia coli/Shigella |
Patient
Request Form
For clients using the Enfer Medical Client Portal, the Enfer Medical Patient Request Form (PRF) will be generated when you have completed your “Test Request” within the portal linked below.
Please note that samples suspected of containing high risk pathogens such as Mycobacterium tuberculosis should be clearly identified on the form and packed separately from other samples.
Results are available next working day.
This assay is available seven days a week.
Packaging
The packaging/preparation of samples for delivery to the laboratory are required to be in adherence to all national regulations for the safe transport of biological materials. HSE guidance can be reviewed HERE.
There are specific packaging instructions and labelling requirements requiring triple packaging including:
- Primary leak-proof container – tube or vial containing the sample.
- Secondary watertight container, with absorbent material, intended to protect the primary container.
- Outer container protects the secondary container.
- Patient Request forms must be placed between the secondary container and the outer shipping container.
Further information on the packaging/preparation of samples for delivery to the laboratory is contained within the Enfer Medical Laboratory User Manual which clients are provided with.
Storage & Stability
Samples must be tested within 4 hours of collection if stored at room temperature. Samples must be tested within 3 days of collection if stored at 2-8°C.
Samples
In addition to the packaging of samples, the transport/delivery of clinical samples to the laboratory is required to be in adherence to all national regulations for the safe transport of biological materials. Enfer Medical provides a fully compliant logistics service, aligned with clients’ testing requirements.
Prompt transport of specimens:
To ensure prompt testing of samples and release of results within the published test turnaround times, samples should arrive to the laboratory by 18:00. Test turnaround time is measured from receipt of the sample at the laboratory until the time the authorised results are reported to users.
of Samples
In rare cases specimens may not be suitable for testing on arrival to the laboratory. In that case the sample will be rejected at the receipt stage and the service user will be notified immediately and an explanation as to why the sample could not be processed will be provided. Reasons why samples cannot be processed include but are not limited to:
- Samples received beyond the stability limits and/or not at the correct temperature indicated for each test.
- Incorrect sample type received.
- Leaking samples, sample not received or sample insufficient for analysis as stated below within sample requirements.
- Non-compliant samples or request forms i.e. those missing sample date information, missing sample test request and/or missing sample site/type information.
- Samples received without the necessary patient identifiers.
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- DETECTED
- NOT DETECTED
- INVALID
Please refer to the Health Protection Surveillance Centre for the Case Definitions for Notifiable Diseases:
Accessing
Your Results
Following receipt at the Laboratory, results should be available on the next working day and will be reported to the referring clinician via the Enfer Medical Client Portal or in a format agreed with clients.
Established customers can login to the portal using the link above.