In Ireland, demand for infertility services has grown substantially in recent times even though the prevalence of infertility has been stable. The HSE estimates 1 in 6 heterosexual couples in Ireland experience infertility. Globally 2.5 million assisted cycles are undertaken per year, with 500,000 babies born as a result. Hormonal analysis is an essential first step in the assessment of the sub-fertile couple, due to ease of access and as a non invasive investigation which can be hugely informative in determining the source of their difficulty in conceiving.