Infection predominantly affects children but it can also be a cause of diarrhoea in the elderly living in long term residential care facilities as well as individuals with immunocompromised conditions. In Ireland it is the most common cause of gastroenteritis in children under the age of 5 and is most prevalent during the Winter and Spring.
Rotavirus A RNA
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Rotavirus A RNA
Rotavirus is an RNA virus of the Reoviridae family with several species identified with Rotavirus A being responsible for the majority of human infections.
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Clinical Details
Infection may be asymptomatic or characterised by vomiting and watery diarrhoea for 3-8 days. Other common symptoms include fever, abdominal pain, loss of appetite, and dehydration. The incubation period of Rotavirus disease is approximately 1-4 days. The primary mode of transmission is by the faecal-oral route by direct person to person contact or from contact with contaminated food, water or environment. Viral shedding can occur up to 3 days after symptoms resolve. Immunity is long lasting following infection.
Two rotavirus vaccines are currently available. For further information please refer to the HSE website.
Case Study
Delivering True Innovation
At Enfer Medical, we have integrated a cutting-edge and fully automated WASPLab® (Walk Away Specimen Processor) that has significantly enhanced our testing capabilities to ensure the highest of quality when processing patient samples.
Our Services
Service
Health & Wellness
General health and wellness testing can include a variety of tests, depending on the needs of the individual or recommendations of
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Service
Sexual Health
Regular STI screening facilitates early treatment and reduces the long-term consequences of infection.
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Service
Respiratory Health
Rapid early pathogen identification has important treatment and infection control implications. See our range of tests here.
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Service
Reproductive & Female Health
Hormonal analysis is hugely beneficial to identifying problems that can arise during the reproductive years.
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Sample Type &
Volume Required
Loose/liquid human faecal sample.
Enfer Medical
Tests/Assays
The test for Rotavirus offered by Enfer Medical is carried out by real-time multiplexed PCR, as part of an extended gastrointestinal panel. The test is CE-IVD certified.
Panel Tests
The pathogens detected on this panel are:
| Bacteria | Viruses | Parasites |
| Campylobacter | Adenovirus F40/41 | Cryptosporidium |
| Clostridium difficile toxin A/B | Astrovirus | Cyclospora cayetanensis |
| Plesiomonas shigelloides | Rotavirus A | Entamoeba histolytica |
| Salmonella | Novovirus GI/GII | Giardia lamblia |
| Vibrio | Sapovirus (Genogroups I, II, IV and V) | |
| Yersinia enterocolitica | ||
| Escherichia coli/Shigella |
Patient
Request Form
For clients using the Enfer Medical Client Portal, the Enfer Medical Patient Request Form (PRF) will be generated when you have completed your “Test Request” within the portal linked below.
Please note that samples suspected of containing high risk pathogens such as Mycobacterium tuberculosis should be clearly identified on the form and packed separately from other samples.
24 hours
Results are available next working day.
7 Days a Week
This assay is available seven days a week.
Packaging
The packaging/preparation of samples for delivery to the laboratory are required to be in adherence to all national regulations for the safe transport of biological materials. HSE guidance can be reviewed HERE.
There are specific packaging instructions and labelling requirements requiring triple packaging including:
- Primary leak-proof container – tube or vial containing the sample.
- Secondary watertight container, with absorbent material, intended to protect the primary container.
- Outer container protects the secondary container.
- Patient Request forms must be placed between the secondary container and the outer shipping container.
Further information on the packaging/preparation of samples for delivery to the laboratory is contained within the Enfer Medical Laboratory User Manual which clients are provided with.
Storage & Stability
Samples must be tested within 4 hours of collection if stored at room temperature. Samples must be tested within 3 days of collection if stored at 2-8°C.
Transporting
Samples
Samples
In addition to the packaging of samples, the transport/delivery of clinical samples to the laboratory is required to be in adherence to all national regulations for the safe transport of biological materials. Enfer Medical provides a fully compliant logistics service, aligned with clients’ testing requirements.
Prompt transport of specimens:
To ensure prompt testing of samples and release of results within the published test turnaround times, samples should arrive to the laboratory by 18:00. Test turnaround time is measured from receipt of the sample at the laboratory until the time the authorised results are reported to users.
Acceptance/Rejection
of Samples
of Samples
In rare cases specimens may not be suitable for testing on arrival to the laboratory. In that case the sample will be rejected at the receipt stage and the service user will be notified immediately and an explanation as to why the sample could not be processed will be provided. Reasons why samples cannot be processed include but are not limited to:
- Samples received beyond the stability limits and/or not at the correct temperature indicated for each test.
- Incorrect sample type received.
- Leaking samples, sample not received or sample insufficient for analysis as stated below within sample requirements.
- Non-compliant samples or request forms i.e. those missing sample date information, missing sample test request and/or missing sample site/type information.
- Samples received without the necessary patient identifiers.
Clients will be provided with guidance on Sample Receipt Anomalies.
Our Services
Service
Health & Wellness
General health and wellness testing can include a variety of tests, depending on the needs of the individual or recommendations of
Find Out More
Service
Sexual Health
Regular STI screening facilitates early treatment and reduces the long-term consequences of infection.
Find Out More
Service
Respiratory Health
Rapid early pathogen identification has important treatment and infection control implications. See our range of tests here.
Find Out More
Service
Reproductive & Female Health
Hormonal analysis is hugely beneficial to identifying problems that can arise during the reproductive years.
Find Out More
Result Values:
Qualitative results will be reported for each pathogen as:
Qualitative results
- DETECTED
- NOT DETECTED
- INVALID
Please note this is a notifiable disease under the Infectious Diseases (Amendment) Regulations 2020 (S.I. No. 53 of 2020) February 2020.
Please refer to the Health Protection Surveillance Centre for the Case Definitions for Notifiable Diseases:
Accessing
Your Results
Following receipt at the Laboratory, results should be available on the next working day and will be reported to the referring clinician via the Enfer Medical Client Portal or in a format agreed with clients.
Established clients can login to the portal using the link above.